Inoculation

MEDIA PREPARATION FOR INNOCULATION OF SACCHAROMYCES CEREVISIAE

After Mr. Shaman gave us some explanations about the components of the bioreactor and their functions, we had move to second part of our lab which was the preparation of media for the innoculation of Saccharomyces Cerevisiae, which is the yeast that we will use in this experiment.

The media that we used in this experiment was YEPG medium which contain yeast extract (1%), Peptone (2%) and glucose (2%). The total medium that needed for the innoculation was 200ml, so the weight for yeast extract, peptone and glucose needed were :

Yeast Extract (1%) = 2g

Peptone (2%) = 4g

Glucose (2%) = 4g

and after that we dissolved them with distilled water.

Image 1: medium of innoculation

After the medium was done, the media was then autoclaved before the innoculation of the yeast step was taken.

After 3o minutes we prepared seed culture, we using sterile toothpicks to scrape up a single colony aseptically. The work will be completed by transferring the colony into the shake flask which contained medium. We left it overnight (18-24 hours) in the incubator shaker.