Parts of a bioreactor

       In the first day meet Mr.Shamam from HT Company at room 148,  he was introduced all compartments of bioreactor. All this part is very important to maintain and standardize the fermentation process for small and large volume production. Below shows a list of compartments of the bioreactor and its functions that was introduced by Mr.Shamam.

 Image 1: compartments inside the vessel of bioreactor

         The function of the vessel is where the fermentation take place and inside it has many of compartments to complete the fermentation process.

1. Head plate:
to cover the top of vessel of bioreactor

2. Antifoam probe:
to prevent contamination in fermentation process

3. Stirrer shaft seal:
To hold the stirrer and head plate

4. Acid/base probe:
A flow to add acid and bases during fermentation process

5. Shaft:
Not allow the medium to escape or microorganism enter

6. 2.5 glass jar bioreactor:
Material of the vessel of bioreactor

7. Baffles:
To prevent a whirl pool effect that could impede proper mixing
per mixing
8. Sampling port:
A valve to get the sample of the fermentation

9. 6- bladed disk impeller:  
a. To diminish the size of air bubbles to give a bigger interfacial area for O₂ transfer and to decrease diffusion path.

b. To maintain uniform environment throughout the vessel content.

10. Air sparger:
To ensure better dispersal of air.

                           Image 2:  All fittings on head plate of the bioreactor

                                                      Image 3: rotameter

 The rotameter is the means of manually controlling the amount of air entering the vessel and for controlling the gas flow rate.

Image 4: holder of reagent bottles

        The function of this holder is where the reagent bottles are usually placed during  

         the fermentation.

                                                 Image 5: reagent bottles

             The reagent bottles that usually needed during the fermentation take place are, reagent bottle for base, antifoam and acid, but in this experiment we do not need acid because we dealing with microorganism, which is yeast (Saccharomyces Cerevisiae) which usually will produce acid trough out the process.

                                                   Image 6: pO2 electrode

            The pO2 electrode should already be connected to the bioreactor base unit which has been powered for at least 2 hours to ensure the electrode is polarized.

 

                                             Image 7: Air compressor

    The function of air compressor  is used to provide air during fermentation process.

 Thats all for the introducing session with bioreactor compartments. Then, we are ready to go the experiment operation.